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Schemes

DNA Extractions

The implementation of whole genome sequencing and next generation sequencing panels requires high quality DNA to allow good sequencing data to be obtained. K NEQAS for Molecular Genetics has been delivering bespoke EQA schemes for the UK 100,000 Genomes Project to assess the standard of DNA samples extracted by laboratories supplying samples for inclusion into the project.
 The Scheme is delighted to now offer participation in these EQAs to all interested laboratories. These EQAs provide laboratories with a measure of the quality of the DNA extracted in-house and enables benchmarking with other centres using similar extraction methods.
 Laboratories linked to a NHS Genomic Medicine Centre do not need to register for participation in the Blood or Tissue DNA extraction schemes. Registration is automatic and the participation fees with be covered by NHS England.
 
DNA EXTRACTION FROM BLOOD SAMPLES
There will be two EQA runs provided during 2017 to assess the quality and quantity of DNA extracted from blood samples. For each run, three peripheral blood samples anticoagulated in EDTA (ranging from 1ml to 5ml) will be distributed to participants. Laboratories will be required to extract DNA from the total volume of blood and return the DNA to the Scheme for quality assessment. The DNA samples will be assessed for total volume, total amount of DNA, level of fragmentation and library preparations made which will be assessed using a bioanalyser. Each sample will be given a quality rating. Overall comparisons between methods will be provided.
The cost of participation will be £1,000 for participation in one EQA run.
 
DNA EXTRACTION FROM TISSUE SAMPLES
During 2017 there will be two EQA runs to assess the quality and quantity of DNA extracted from tissue samples. This will include formalin fixed paraffin embedded (FFPE) tissue and fresh frozen tissue. Laboratories will be required to extract DNA from the total sample supplied and return the DNA to the Scheme for quality assessment. he DNA samples will be assessed for total volume, total amount of DNA, level of fragmentation using commercial kits. Each sample will be given a quality rating. Overall comparisons between methods will be provided.
The cost of participation will be as follows:
£400 for one EQA run for DNA extraction from FFPE tissue
The fee for one EQA run for DNA extraction from  fresh frozen tissue is to be finalised.
 
DNA EXTRACTION FROM SALIVA SAMPLES
During 2017 there will be two EQA runs to assess the quality and quantity of DNA extracted from saliva samples. For each run, three saliva samples (ranging from 0.5ml to 2ml) will be distributed to participants. Laboratories will be required to extract DNA from the total sample supplied and return the DNA to the Scheme for quality assessment.  The DNA samples will be assessed for total volume, total amount of DNA, level of fragmentation and library preparations made which will be assessed using a bioanalyser. Each sample will be given a quality rating. Overall comparisons between methods will be provided.
The cost of participation will be £500 for participation in one EQA run (subsidised).